Transferable Resistance to 3 rd Generation Cephalosporins andAztreonam in Strains of Klebsiella pneumoniae Producing b-lactamases with anExtended Spectrum (ESBL)
Prenosná rezistencia na cefalosporíny 3. generáciea aztreonam u kmeňov Klebsiella pneumoniaeprodukujúcich b-laktamázy s rozšíreným spektromúčinku (ESBL)
Autori demonštrovali transferabilitu génov antibiotickej rezistencie u nozokomiálnych kmeňovKlebsiella pneumoniae, izolovaných od novorodencov v Detskej fakultnej nemocnici s poliklinikouv Bratislave, rezistentných na cefotaxím, ceftazidím a aztreonam. Determinanty rezistencie nab-laktámové antibiotiká (karbenicilín, cefaloridín, cefotaxím, ceftazidím a aztreonam) boli prenosnéna recipientné kmene Escherichia coli K-12 a Proteus mirabilis P-38. Prenos determinantov rezis-tencie z donorných kmeňov bol dokázaný analýzou spektra rezistencie u transkonjugantných klo-nov recipientných kmeňov metódou nepriamej selekcie. Pomocou dvojdiskového difúzneho testubola u týchto kmeňov zistená schopnosť produkovať ESBL (b-laktamázy s rozšíreným spektromúčinku). Synergia medzi klavulanátom a cefotaxímom, klavulanátom a ceftazidímom, alebo klavu-lanátom a aztreonamom indikovala produkciu ESBL týmito kmeňmi.
Klíčová slova:
Klebsiella pneumoniae – b-laktamázy s rozšíreným spektrom účinku – transferabilnárezistencia.
Authors:
M. Babálová 1; J. Blahová 1; K. Králiková 1; V. Krčméry 1; J. Hanzen 2; O. Balogová 2; P. Milošovič 2
Authors‘ workplace:
Ústav preventívnej a klinickej medicíny, Bratislava2 HPL s. r. o., mikrobiologické laboratórium, Bratislava
1
Published in:
Epidemiol. Mikrobiol. Imunol. , 1999, č. 1, s. 21-27
Category:
Overview
The authors demonstrated the transferability of antibiotic resistance genes in nosocomial strainsof Klebsiella pneumoniae, isolated from newborn children at the Paediatric University Hospital inBratislava. Strains were resistant to cefotaxime, ceftazidime and aztreonam. The determinants ofresistance (carbenicillin, cephaloridine, cefotaxime, ceftazidime and aztreonam) were transferredto recipient strains of Escherichia coli K-12 and Proteus mirabilis P-38. The transfer of resistancedeterminant from donor strains was demonstrated by the analysis of the resistance spectrum intransconjugant clones of recipient strains by the method of indirect selection. The ability ofproduction of extended spectrum b-lactamases (ESBL) was demonstrated by the double disc diffu-sion test. Synergy between clavulanate and cefotaxime, clavulanate and ceftazidime and/or clavu-lanate and aztreonam indicated production of ESBL by these strains.
Key words:
Klebsiella pneumoniae – extended-spectrum b b-lactamase – transferable resistance.
Labels
Hygiene and epidemiology Medical virology Clinical microbiologyArticle was published in
Epidemiology, Microbiology, Immunology
1999 Issue 1
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