Effect of Nickel Sulphate, Potassium Dichromate, Cobalt Nitrate and CadmiumSulphate on Expression of ICAM-1 and HLA-DR Molecules on Cultivated HumanKeratinocytes
Vplyv nikelsulfátu, káliumdichromátu, kobaltnitrátua kadmiumsulfátu na expresiu ICAM-1 a HLA-DRmolekúl na kultivovaných ľudských keratinocytoch
Autori testovali schopnosť známych alergénov nikelsulfátu, káliumdichromátu a kobaltnitrátuindukovať expresiu povrchových adhezívnych molekúl ICAM-1 a HLA-DR na kultivovaných ľudskýchkeratinocytoch v porovnaní s cytotoxickým kadmiumsulfátom.Na sledovanie expresie povrchových molekúl použili metódu priamej imunofluorescencie a imunoprecipitácie.Zaznamenali len slabú, nepravidelnú expresiu ICAM-1 po pôsobení nikelsulfátu,káliumdichromát, kobaltnitrát a kadmiumsulfát expresiu ICAM-1 neindukovali. Žiaden z testovanýchalergénov nevyvolal indukciu expresie HLA-DR molekuly.Záver: Pri použití uvedených metodík nie je sledovanie expresie molekúl ICAM-1 a HLA-DR nakultivovaných ľudských keratinocytoch preukazným markerom na testovanie možného alergizujúcehopotenciálu chemických látok.
Klíčová slova:
kultivované ľudské keratinocyty - povrchové adhezívne molekuly ICAM-1,HLA-DR- alergény - testovanie
Authors:
A. Lozseková 1; Kaiser H.-W. 2; T. Danilla 3; J. Buchvald 1; J. Šimko 4
Authors‘ workplace:
I. Kožná klinika LF UK a FN, Bratislava, prednosta prof. MUDr. Jozef Buchvald, DrSc. 2Universitäts-Hautklinik, Bonn, prednosta prof. Dr. med. Hans-Wilhelm Kreysel 3Detská kožná klinika DFNsP, Bratislava, prednosta doc. MUDr. Tibor Danilla, CSc. 4II. gynek
1
Published in:
Čes-slov Derm, , 2003, No. 3, p. 105-110
Category:
Overview
The authors tested the capacity of known allergens nickel sulphate, potassium dichromate andcobalt nitrate to induce expression of surface adhesive molecules ICAM-1 and HLA-DR on cultivatedhuman keratinocytes as compared with cytotoxic cadmium sulphate.To test expression of surface molecules they used the method of direct immunofluorescence andimmunoprecipitation. They recorded only a weak irregular expression of ICAM-1 after the action ofnickel sulphate. Potassium dichromate, cobalt nitrate and cadmium sulphate did not induce ICAM-1expression. None of the tested allergens induced expression of the HLA-DR molecule.Conclusion: When using the above methods, assessment of expression of ICAM-1 and HLA-DRmolecules on cultivated human keratinocytes is not a reliable marker for testing the possibleallergizing potential of chemical substances.
Key words:
cultivated human keratinocytes - adhesive surface molecules ICAM-1 - HLA-DR -allergens - testing
Labels
Dermatology & STDs Paediatric dermatology & STDsArticle was published in
Czech-Slovak Dermatology
2003 Issue 3
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