Quantitative analysis of chimerism after allogenichematopoietic cells transplantation
Kvantitativní analýza chimerismu po alogennítransplantaci hematopoetických buněk
Vyšetření chimerismu se stalo nedílnou součástí sledování pacientů po alogenní transplantaci krvetvornýchbuněk. Předkládaná publikace nejprve seznamuje s různými metodami jeho stanovení.Ve druhé části je pak detailně uvedena charakteristika metody kapilární elektroforézy s fluorescenčnídetekcí (fragmentační analýzy) produktů amplifikace vysoce polymorfních mikro- a mini- satelitníchoblastí genomové DNA, metody, která je v současnosti považována za „gold standard“ metodu stanovení.
Klíčová slova:
chimerismus, STR a VNTR polymorfismy, fragmentační analýza
Authors:
J. Dušková; Z. Broukal
Authors‘ workplace:
Centrum molekulární biologie a genové terapie, Interní hematoonkologická klinika, FN Brno
Published in:
Prakt. Lék. 2004; (10): 70-75
Category:
Overview
Examination of chimerism has become an integral element of the monitoring of patients after allogenichematopoietic cell transplantation. Presented publication initially describes different methods ofchimerism evaluation. In the second part, there is detailed characterization of the capillary electrophoresiswith fluorescence detection (fragment analysis) of amplification products of high polymorficmicro- and mini- satellite DNA regions, technique, which become the gold standard for quantitativeevaluation.
Key words:
chimerism, STR and VNTR polymorphism, fragment analysis
Labels
General practitioner for children and adolescents General practitioner for adultsArticle was published in
General Practitioner
2004 Issue 10
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