Comparison of Some Extraction Methods for the Detection of EhrlichianDNA in Full Blood by Means of PCR
Srovnání některých extrakčních postupů pro průkazehrlichiové DNA metodou PCR v plné krvi
Bylo provedeno srovnání devíti metod izolace specifické DNA etiologického agens humánní granulocytárníehrlichiózy z lidské krve pro účely vyšetření PCR. Do plné krve zdravého dárce byladefinovaně přimíšena laboratorní kultura agens a testována účinnost izolace a stabilita templátuza podmínek, kdy byla krev čerstvá nebo zmražená. K univerzálnímu použití byl nejvhodnějšíQIAamp® DNA Mini Kit (QIAGEN), zmražená krev byla nejlépe extrahována pomocí Nucleo SpinTissue (Macherey-Nagel).
Klíčová slova:
humánní granulocytární ehrlichióza (HGE) – Anaplasma phagocytophila – polymerázovářetězová reakce (PCR) – extrakce DNA.
Authors:
P. Zeman
Authors‘ workplace:
KHS Středočeského kraje, Praha
Published in:
Epidemiol. Mikrobiol. Imunol. , 2002, č. 4, s. 143-147
Category:
Overview
The author compared nine methods for isolation of specific DNA of the etiological agent of humangranulocytic ehrlichiosis from human blood for examination of the PCR. To full blood of healthydonors a laboratory culture of the agent was added and the effectiveness of isolation an stability ofthe template was tested under conditions when the blood was fresh or frozen. For universal useQIAamp® (QIAGEN) was most suitable, frozen blood was extracted best using NucleoSpin® Tissue(Macherey-Nagel).
Key words:
human granulocytic ehrlichiosis (HGE) – Anaplasma phagocytophila – polymerase chainreaction (PCR) – DNA extraction.
Labels
Hygiene and epidemiology Medical virology Clinical microbiologyArticle was published in
Epidemiology, Microbiology, Immunology
2002 Issue 4
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