Brassica oleracea in vitro
Brassica oleracea in vitro
Byla založena tkáňová kultura. Nejvyšších přírůstků čerstvé hmotnosti kultur bylo dosaženo na médiích s přídavkem IBA, NAA a IAA v koncentraci 10,0 mg.l -1. Rozdíly mezi hodnotami na uvedených médiích byly statisticky nevýznamné. V extraktech kalusů byly bez ohledu na hormo- nální složení média prokázány sacharidy (sacharóza, fruktóza), z terpenoidů b-sitosterol. Obsah aminokyselin byl ovlivněn hormonálním složením média. V porovnání s intaktní rostlinou se vyskytly rozdíly v kvalitativním i kvantitativním zastoupení aminokyselin. Biotransformační po- kusy prokázaly schopnost kultury biotransformovat hydrochinon na arbutin. Bylo dosaženo úplné regenerace celých rostlin organogenezí.
Klíčová slova:
tkáňová kultura – Brassica oleracea – aminokyseliny – biotransformace – regenerace
Authors:
J. Dušková; L. Jahodář; J. Dušek 1
Authors‘ workplace:
Katedra farmaceutické botaniky a ekologie, Farmaceutické fakulty Univerzity Karlovy, Hradec Králové 1 Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové
Published in:
Čes. slov. Farm., 1998; , 264-267
Category:
Overview
A tissue culture was established. Highest increases in the fresh weight of cultures were achieved on the media with additions of IBA, NAA and IAA in a concentration of 10.0 mg.l -1. Differences in the values on the above-mentioned media were statistically insignificant. In callus extracts irres- pective of the hormonal composition of the medium, saccharides (saccharose, fructose) and the terpenoid b-sitosterol were demonstrated. The content of amino acids was influenced by the hormonal composition of the medium. In comparison with the intact plant, there occurred differences in both qualitative and quantitative representation of amino acids. Biotransformational experi- ments demonstrated an ability of the culture to biotransform hydroquinone to arbutin. Complete regeneration of whole plants by organogenesis was achieved.
Key words:
tissue culture – Brassica oleracea – amino acids – biotransformation – regeneration
Labels
Pharmacy Clinical pharmacologyArticle was published in
Czech and Slovak Pharmacy
1998 Issue 6
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